Pharmacokinetics of Puerarin Self-microemulsions in Rats

Objective: To estabolish a quantitative analysis method for pharmacokinetics and bioavailability of puerarin self-microemulsions in rats. Method: Puerarin self-microemulsions and suspensions were given by a single dose,HPLC was adopted to determine the concentration of puerarin in plasma with detection wavelength at 250 nm and mobile phase of methanol(A)-0. 1% phosphoric acid solution(B) for gradient elution(0-30 min,20%-25% A; 30-40 min,25%-40% A; 40-50 min,40% A),pharmacokinetics parameters and bioavailability was calculated by DAS 2. 1. 1 programs. Result: tmaxof puerarin self-microemulsions and suspensions were 0. 71,1. 1 h,Cmaxwere 2. 052,1. 120 mg·L-1,AUC0-24 hwere 2. 901,2. 013 mg·h·L-1,respectively. Relative bioavailability of puerarin self-microemulsions to suspensions was 144. 11%. Conclusion: Compared with puerarin suspensions,puerarin self-microemulsion could significantly improve bioavailability in rats.