Cloning of Pollen Fertility Gene MS2 and Construction of Its RNAi Expression Vector

[Objective]The aim was to study the function of MS2 gene.[Method] Cotton pollen fertility(MS2) gene cDNA fragment was cloned by RT-PCR;the senseMS2gene and antisense cDNAs of MS2 gene were ligated with the first intron of cotton chinase gene;the three fragments-fused sequence was identified and inserted into the plant expression vector of PBI121.[Result] The RNAi vector P35S12MSIn of MS2 gene was successfully constructed.[Conclusion]The vector created the conditions for genetic transformation in cotton sterile material.