Genotyping analysis in human papillomavirus infection

Objective Analyse the genotypes of human papillomavirus in clinical cases with papillomavirus infection. Methods 213 high risk HPV positive samples screened by Digene Hybrid Capture II were genotyped by PCR/Reversed dot blot hybridization. Results 195 high risk HPV positive cases could be genotyped specifically by PCR/RDB. 17 high risk and low risk HPV genotypes were detected, namely HPV 16(32.3%)、HPV 52(23.1%)、HPV 58(18.0%)、HPV 31(11.8%)、HPV 68(10.3%)、HPV 33(9.7%)、HPV 53(8.2%)、 HPV 18(8.2%)、HPV 66(6.2%)、HPV CP8304(4.6%)、HPV6(3.6%)、HPV59(3.1%) HPV45(2.1%)、HPV39(2.1%)、HPV11(2.1%)、HPV56(1.5%)、HPV51(1.0%). Among the 195 HPV infected samples, the detection rates of 1 HPV genotype infection, 2 HPV genotypes infection, 3 and 4 HPV genotypes infection were 59.5%, 30.8%, 8.6%, 1.1%, respectively. Conclusion The major genotypic prevalence in high risk HPV infection was HPV 16, HPV 52 and HPV 58. The HPV 56 and HPV 51 had less detection rate. HPV 58 had lower detection rate in other parts of the world whereas there was high incidence in China. Single HPV genotype was key HPV infection. Mixed infection of more than two HPV genotypes should be paid more attention.