Analysis of Genetic Subtypes detection of Human Papillomavirus in cervical cells of 1000 women

Objective To investigate the status of HPV infection and genotypes distribution of HPV of women in Shenzhen.Methods The samples of cervial cells were detected by HPV PCR reverse dot blot hybridization genotyping in 1000 cases.The infection rate,genotype and age distribution in the female were analyzed.Results The total infection rate of HPV in 1000 cases was 24.5%.HPV44,HPV83 and HPVMM4 were not detected in 20 genetypes.The top three low-risk HPV genotypes were HPV6(9.71%),HPV11(8.63%) and HPV43(7.19%),while the top four high-risk HPV genotypes were HPV16(16.19%),HPV52(11.87%),HPV58(10.43%) and HPV66(9.35%).The infection rate of HPV gene subtypes in the low-risk groups was lower than that in the high-risk groups.HPV detection rate in 56-65 years-old group was 31.91%,which was the highest in the total five groups.Single HPV genotype was positive in 176 cases(17.60%),two HPV genotypes were positive in 52 cases(5.20%),three HPV genotypes were positive in 13 cases(0.13%),and more than four HPV genotypes were positive in 4 cases(0.04%).Conclusion The infection rate of HPV is 24.5%,single gene infection is the main subtype,which subtypes is type 16,type 52 and type 58,and detection rate in 56-65 years group was the highest in the total five groups.The detection of HPV-DNA is of great important in the early prevetion,diagnosis and treatment of cervical cancer.