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2005•Journal of Chemical Industry and EngineeringRequires access

Flexible immobilization of papain on amino polystyrene support

Ouyang Pingkai

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Abstract

In this paper the flexible immobilization model of enzyme is presented.A new amino polystyrene (0.4—6.0 mmol NH_2·g -1) was prepared by Mannich reaction as immobilization support, DAS was used as the flexible chain,and then papain was immobilized. The recovery of immobilized enzyme activity could reach 40%—50%,equal to 1.8—2.4 times that of arm spacer support, and the stability of immobilized enzyme was good.The flexible immobilized model could achieve a higher recovery of immobilized enzyme activity than traditional covalent immobilization and arm spacer immobilization.

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What this paper is about

In this paper the flexible immobilization model of enzyme is presented.A new amino polystyrene (0.4—6.0 mmol NH_2·g -1) was prepared by Mannich reaction as immobilization support, DAS was used as the flexible chain,and then papain was immobilized. The recovery of immobilized enzyme activity could reach 40%—50%,equal to 1.8—2.4 times that of arm spacer support, and the stability of immobilized enzyme was good.The flexible immobilized model could achieve a higher recovery of immobilized enzyme activity than traditional covalent immobilization and arm spacer immobilization.

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Available abstract

In this paper the flexible immobilization model of enzyme is presented.A new amino polystyrene (0.4—6.0 mmol NH_2·g -1) was prepared by Mannich reaction as immobilization support, DAS was used as the flexible chain,and then papain was immobilized. The recovery of immobilized enzyme activity could reach 40%—50%,equal to 1.8—2.4 times that of arm spacer support, and the stability of immobilized enzyme was good.The flexible immobilized model could achieve a higher recovery of immobilized enzyme activity than traditional covalent immobilization and arm spacer immobilization.

Key concepts: Papain, Immobilized enzyme, Chemistry, Covalent bond, Polystyrene, Chromatography, Enzyme, Polymer chemistry

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