Flexible Immobilized Papain on the Chitosan Support

In this work 揊lexible Immobilized Enzyme?model is adopted. Chitosan-DAS (Dialdehyde Starch) flexible support is synthesized by coupling flexible chain (DAS) onto the surface of chitosan support, then papain is immobilized on this flexible support. Through optimizing immobilization conditions, it is found that when Chitosan-DAS50 is taken as support with the conditions of 14.4 mg/g (enzyme/dry support) and pH 8, immobilization time is 18 h, the recovery of immobilized enzyme activity can reach 72%, being equal to triple of Chitosan-GA (glutaric aldehyde) support. This conclusion has showed that 揊lexible Immobilized Enzyme?model can improve recovery of immobilized enzyme activity over traditional covalent immobilization and arm spacer immobilization.