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2007•Journal of Forensic MedicineRequires access

Establishment of A PC12 Cell Injury Model Induced by H_2O_2

Jing Huan-lan

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Abstract

Objective To establish an apoptosis model of PC12 cell with different concentration H2O2 and to study the mechanism of tissue ischemia and free radical injury. Methods After 12 hour treatment with different concentrations of H2O2, the viability and apoptosis of PC12 cells were determined by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) and flow cytomertry (FCM) methods. Results The viability of PC12 cells induced by 100 nmol/L and 300 nmol/L H2O2 was (76.00±7.69)% and (39.00±7.08)%, respectively, compared to the 0 nmol/L group (P0.01). The apoptosis ratio in PC12 cells induced by 0 nmol/L, 100 nmol/L, and 300 nmol/L H2O2 were 2.47%, 6.00%, and 55.54%, respectively. Conclusion A PC12 cell injury model induced by different concentrations of H2O2 for the study of apoptosis was successfully established.

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Objective To establish an apoptosis model of PC12 cell with different concentration H2O2 and to study the mechanism of tissue ischemia and free radical injury. Methods After 12 hour treatment with different concentrations of H2O2, the viability and apoptosis of PC12 cells were determined by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) and flow cytomertry (FCM) methods. Results The viability of PC12 cells induced by 100 nmol/L and 300 nmol/L H2O2 was (76.00±7.69)% and (39.00±7.08)%, respectively, compared to the 0 nmol/L group (P0.01). The apoptosis ratio in PC12 cells induced by 0 nmol/L, 100 nmol/L, and 300 nmol/L H2O2 were 2.47%, 6.00%, and 55.54%, respectively. Conclusion A PC12 cell injury model induced by different concentrations of H2O2 for the study of apoptosis was successfully established.

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Available abstract

Objective To establish an apoptosis model of PC12 cell with different concentration H2O2 and to study the mechanism of tissue ischemia and free radical injury. Methods After 12 hour treatment with different concentrations of H2O2, the viability and apoptosis of PC12 cells were determined by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) and flow cytomertry (FCM) methods. Results The viability of PC12 cells induced by 100 nmol/L and 300 nmol/L H2O2 was (76.00±7.69)% and (39.00±7.08)%, respectively, compared to the 0 nmol/L group (P0.01). The apoptosis ratio in PC12 cells induced by 0 nmol/L, 100 nmol/L, and 300 nmol/L H2O2 were 2.47%, 6.00%, and 55.54%, respectively. Conclusion A PC12 cell injury model induced by different concentrations of H2O2 for the study of apoptosis was successfully established.

Key concepts: Apoptosis, Viability assay, Chemistry, MTT assay, Flow cytometry, Molecular biology, Andrology, Pharmacology

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