Effect of apoB gene G12669A polymorphism on plasma lipid levels of Changsha Han Chinese with cerebral hemorrhage

【Objective】To explore the effect of apolipoprotein B(apoB) gene G12669A polymorphism on plasma lipid levels of Changsha Han Chinese with cerebral hemorrhage.【Methods】Peripheral blood specimens were collected from 93 cerebral hemorrhage patients and 100 normal control people.The levels of TG,TC,HDL and LDL were examined by oxidase method.The serum levels of LP(a),apoB-100 and apoAI were determined by immune methods.The G12669A polymorphism of apoB was analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-PFLP) and direct sequencing.【Results】①TC,TG,LDL-C and LP(a) levels of Changsha Han Chinese with cerebral hemorrhage were significantly higher than those of control group,while the level of HDL-C was significantly lower(P0.05).②In CH patients group,the levels of TC,TG and LDL-C in the people with G/A genotype were significantly higher than those in the people with G/G genotype,while the level of HDL-C was significantly lower(P0.05).③A allele frequencies of apoB G12669A in cerebral hemorrhage patients and the control group were 0.097 and 0.045 respectively.A allele frequency of apoB G12669A in CH patients was significantly higher than that in the control group(P0.05).【Conclusion】Lipid levels of cerebral hemorrhage patients in Changsha Han Chinese are significantly higher than in control group(P0.05),which may be related with A allele of apoB G12669A polymorphism.