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2006•Journal of Southeast UniversityRequires access

Construction of recombinant plasmid highly expressing luciferase in Drosophila S2 cells

Yuan Liu-di

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Abstract

Objective To construct recombinant plasmid highly expressing luciferase in Drosophila S2 cells.Methods Recombinant plasmid pGL_(3)-pac was constructed by PCR and subcloned and transiently transfected into Drosophila S2 cells.The activity of LUC and β-gal were determined to calculate the RLUC after transfection.Result Compared with pGL_(3)-Control,there was nearly 27 thousand increase in pGL_(3)-pac activity.Conclusion The recombinant plasmid highly expressing luciferase has been successfully constructed in Drosophila S2 cells.

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What this paper is about

Objective To construct recombinant plasmid highly expressing luciferase in Drosophila S2 cells.Methods Recombinant plasmid pGL_(3)-pac was constructed by PCR and subcloned and transiently transfected into Drosophila S2 cells.The activity of LUC and β-gal were determined to calculate the RLUC after transfection.Result Compared with pGL_(3)-Control,there was nearly 27 thousand increase in pGL_(3)-pac activity.Conclusion The recombinant plasmid highly expressing luciferase has been successfully constructed in Drosophila S2 cells.

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Available abstract

Objective To construct recombinant plasmid highly expressing luciferase in Drosophila S2 cells.Methods Recombinant plasmid pGL_(3)-pac was constructed by PCR and subcloned and transiently transfected into Drosophila S2 cells.The activity of LUC and β-gal were determined to calculate the RLUC after transfection.Result Compared with pGL_(3)-Control,there was nearly 27 thousand increase in pGL_(3)-pac activity.Conclusion The recombinant plasmid highly expressing luciferase has been successfully constructed in Drosophila S2 cells.

Key concepts: Luciferase, Recombinant DNA, Plasmid, Transfection, Schneider 2 cells, Molecular biology, Drosophila (subgenus), Biology

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