Construction and Significance of G0S2 Promoter-Directed Luciferase Reporter Gene Plasmid

Objective: To clone the luciferase reporter gene and construct promoter region from human G0S2 gene. Methods: A region containing human G0S2 gene promoter was obtained by PCR amplification from 293A cells, and the segment was cloned into the pGL3-Basic vector and transformed into E.Coli, which was verified by endonuclease, PCR amplification and direct sequencing. The final construct(pGL3-G0S2-Promoter) was transferred into VSMC(vascular smooth muscle cells), and the luciferase activity was measured. Results: The insert and surrounding regions in pGL3-G0S2-Promoter plasmid were confirmed. There was significant promoter activity for pGL3-G0S2-Promoter vector(P0.05). Conclusions: Luciferase reporter plasmid pGL3-G0S2-Promoter containing G0S2 promoter can be built successfully. It will be used to study the transcriptional regulation of G0S2 gene.