CONSTRUCTION AND EUKARYOTIC EXPRESSION OF NS1 PROTEIN OF INFLUENZA A VIRUS SUBTYPE H1N1

[Objective]To construct the full-length NS1 gene of influenza A Virus Subtype H1N1 into a eukaryotic expression vector PXJ40-HA,and study the expression of NS1 gene in transfected 293T cells.[Methods]The NS1 gene of influenza A Virus Subtype H1N1 was amplified by RT-PCR and cloned into pMD18-T vector to construct a plasmid,named pMD18-T-NS1.The pMD18-T-NS1 and the PXJ40-HA were double digested by the same restrict enzymes,the recombinant eukaryotic expression vector PXJ40-HA-NS1 subsequently yielded. The expression of the NS1 gene in transfected 293T cells was tested by Western blot.[Results]The recombinant eukaryotic expression vector PXJ40-HA-NS1 was successfully constructed. The NS1 protein was finally expressed in 293T cells.[Conclusion]The full-length NS1 gene was obtained as well as its recombinant eukaryotic expression plasmid was successfully constructed and expressed in 293T cells. The construction of eukaryotic expression plasmid of NS1 gene made it possible to further study the function of NS1 protein and the mechanism of diseases induced by influenza A virus.