Construction and Expression of NS1 Protein Eukaryotic Expression Vector of Influenza A Virus Subtype H7N9

This study was designed to construct non-structural protein NS1 eukaryotic expression vector of influenza A virus subtype H7N9 and express the NS1 protein in 293 Tcells.The NS1 gene of influenza A virus subtype H7N9 was amplified by RT-PCR and cloned into pcDNA4-Flag-HA vector to construct a plasmid,named pcDNA4-Flag-HA-NS1.The recombinant eukaryotic expression vector pcDNA4-Flag-HA-NS1 subsequently yielded.The expression of the NS1 gene in transfected 293 T cells was tested by Western blotting.The results showed that the recombinant eukaryotic expression vector pcDNA4-Flag-HANS1 was successfully constructed;the NS1 protein was finally expressed in 293T cells;the full-length NS1 gene was obtained as well as its recombinant eukaryotic expression plasmid was successfully constructed and expressed in 293 Tcells.The construction of eukaryotic expression plasmid of NS1 gene was made,and made foundations for further study the function of NS1 protein and the mechanism of diseases induced by influenza A virus.