2009•China Practical MedicineRequires access
Expression of HIV-1 Gag-gp120 chimeric proteins in recombinant baculovirus system
Jin Ning-y
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Abstract
The HIV-1 subtype B gag gene were cloned into baculovirus transfer vector pfastbac1 and the full length of HIV-1 subtype B gp120 gene were colned at the 3/ terminal of gag gene at the same reading frame ,the recombinant plasmid pFge were constructed. After transformation and con-transfecting the recombinant Bacmid with sf 9 cells,the recombinant baculovirus expressed HIV-1 chimric protein were obtained.