Development of RACE assay for amplification of full length sequence of camel β-defensin cDNA

In order to obtain full-length cDNA sequence of camel β-defensin(caBD-1),the 3′cDNA end of caBD-1 was cloned successfully by using anchored PCR-RACE with a specific primer as upstream pri-(mer),which was designed based on the known partial sequence of caBD-1 gene,and a 3′sites adaptor primer as down-stream primer.The 5′cDNA end was amplified rapidly by reverse nested PCR with a specific RT primer,whose 5′end was phosphated,and two specific reverse nested PCR primers.Results revealed that the RACE assays were able to amplify the full-length cDNA of caBD-1.