Pre-clinical Pharmacokinetics of Novel Soybean Iosflavone Sulfonate

Objective To judge the prodrug for novel soybean isoflavone sulfonate(DBS) and study oral bioavailability of daidzein(DZ) in the prodrug. Methods Blood samples were collected 1h after the healthy Wistar rats were iv given DBS 100 mg/kg for prodrug analysis.Samples were collected after Wistar rats were ig or iv given DBS 35 mg·kg-1 at different time points.The DBS and DZ in plasma were determined by HPLC.The compartment model was fitted and pharma cokinetic parameters were calculated by DAS 2.1.1. Results In the drug metabolism experiments,DZ could be detected in rat plasma after oral administration of DBS.The results showed that the pharma cokinetic process of DBS in rats was three compartment model and DZ was one compartment model after rats were ig given DBS 35mg·kg-1.The t1 /2(min) was(219.6±123.6)and(609.6±387.6),respectively.The relative oral bioavailability of DZ in DBS was only 42.9%. Conclusion Although the relative oral bioavailability of DZ in soybean isoflavone sulfonate DBS did not achieve the desired improvement,but DBS was proved to be the prodrug of DZ in this study.It does uesful researches in improving the oral bioavailability of soybean isoflavone.