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Determination of pantoprazole in human plasma by HPLC

Zhao Huai-qing

Open publisher page 1 citations

Abstract

Objective To develop an HPLC method for the determination of pantoprazole in human plasma.Methods After a simple liquid-liquid extraction,pantoprazole and the internal standard were separated on Diamonsil C18 column and detected at the wavelength of 288 nm.The mobile phase consisted of methanol-water(V∶V=60∶40).Results The linear calibration curve was obtained in the concentration range of 0.02-5.0 mg·L-1.The lower limit of quantification was 20 μg·L-1.The intra-and inter-day precision(RSD) were less than 8.4%,and the accuracy was within 104.3% calculated from QC samples.The extraction recoveries of pantoprazole at different levels(low,medium,high) were 75.4%,64.8% and 65.3%,respectively.Conclusions The method is proved to be suitable for the clinical investigation of pharmacokinetics of pantoprazole,which offers the advantage of simplicity,speed and sensitivity.

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What this paper is about

Objective To develop an HPLC method for the determination of pantoprazole in human plasma.Methods After a simple liquid-liquid extraction,pantoprazole and the internal standard were separated on Diamonsil C18 column and detected at the wavelength of 288 nm.The mobile phase consisted of methanol-water(V∶V=60∶40).Results The linear calibration curve was obtained in the concentration range of 0.02-5.0 mg·L-1.The lower limit of quantification was 20 μg·L-1.The intra-and inter-day precision(RSD) were less than 8.4%,and the accuracy was within 104.3% calculated from QC samples.The extraction recoveries of pantoprazole at different levels(low,medium,high) were 75.4%,64.8% and 65.3%,respectively.Conclusions The method is proved to be suitable for the clinical investigation of pharmacokinetics of pantoprazole,which offers the advantage of simplicity,speed and sensitivity.

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Available abstract

Objective To develop an HPLC method for the determination of pantoprazole in human plasma.Methods After a simple liquid-liquid extraction,pantoprazole and the internal standard were separated on Diamonsil C18 column and detected at the wavelength of 288 nm.The mobile phase consisted of methanol-water(V∶V=60∶40).Results The linear calibration curve was obtained in the concentration range of 0.02-5.0 mg·L-1.The lower limit of quantification was 20 μg·L-1.The intra-and inter-day precision(RSD) were less than 8.4%,and the accuracy was within 104.3% calculated from QC samples.The extraction recoveries of pantoprazole at different levels(low,medium,high) were 75.4%,64.8% and 65.3%,respectively.Conclusions The method is proved to be suitable for the clinical investigation of pharmacokinetics of pantoprazole,which offers the advantage of simplicity,speed and sensitivity.

Key concepts: Pantoprazole, Chromatography, Calibration curve, Chemistry, High-performance liquid chromatography, Extraction (chemistry), Human plasma, Detection limit

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