Establishment of Phytolacca acinosa Roxb hairy root in vitro culturing system

Objective:To establish a culture system for hairy root of Phytolacca acinosa Roxb. Methods: The cotyledon explants of Phytolacca acinosa were infected with Agrobacterium rhizogenes strains A4, R1600 and C58C1 and the hairy root culture clones were obtained; the better lines were selected. The growth curve was surveyed and extrinsic factors affecting the growth of hairy roots were investigated. The transformation of Ri tDNA was examined through PCR and high voltage paper electrophoresis. Results: The hairy root was obtained from Phytolacca acinosa. The results of PCR and high voltage paper electrophoresis confirmed the transformation of tDNA from Ri plasmid to the hairy root. Conclusion: The acquisition of hairy root of Phytolacca acinosa lays a foundation for the mass production of active components and introduction of foreign gene.