Establishment of Phytolacca Americana L. Genetic Transformation System by Agrobacterium Rhizogenes and Analysis of Hairy Root Strains Biomass

Objective To establish the genetic transformation system of Phytolacca americana L. by Agrobacterium rhizogenes mediated and analysis of hairy root strains biomass for the base of Phytolacca americana L. research using genetic engineering technology. Methods Activated Agrobacterium rhizogenes R1601,transforming leaves and stems of Phytolacca americana L. by using different concentrations of Agrobacterium rhizogenes R1601; Hairy roots identified by PCR; randomly selected hairy roots to culture,identified the biomass differences of different strains. Results Phytolacca americana L. is sensitive by Agrobacterium rhizogenes R1601 infected; Four concentrations can induce hairy roots of Phytolacca americana L. highly efficient,the genetic transformation efficiency of stems and leaves can reach 100 % if A600 is 1. 0; PCR identification showed the T-DNA sequences had integrated into the hairy roots of Phytolacca americana L.; The biomass analysis showed the biomass of different hairy root strains was significantly different. Conclusion The genetic transformation system highly efficient of Phytolacca americana L. by Agrobacterium rhizogenes has been established,the biomass of different hairy root strains was significantly different.