2010Northern HorticultureRequires access

Effect of Four Kinds of Antibiotics on the Mycelium Growth of Three Edible Fungi of Pleurotus

Zou Jin-mei

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Abstract

The investigations were carried out that effect of four kind antibiotics,including kanamycin,ampicillin,ceftaxime and hygromycin on mycelia growth of three edible fungi Pleurotus species such as Pleurotus eryngii,Pleurotus ostreatus and Pleurotus geesteranus.The results showed that it were no significant influence that cefotaxime,kanamycin and ampicillin against three kind edible mushroom mycelial growth rate and clone morphology,moreover some treatment within the scope of experimental concentration can weakly promote the mycelial growth.Hygromycin strongly inhibited mycelial growth of three pleurotus species,and 12.5 mg/L of hygromycin can completely inhibit the growth of mycelia of P.geesteranus,and it need 50 mg/L hygromycin for achieving the aim of complete inhibition of the mycelia growth of P.eryngii and P.ostreatus.The results laid a foundation of further study on the construction of Agrobacterium Tumefaciens-mediated genetic transformation system of three Pleurotus species.

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The investigations were carried out that effect of four kind antibiotics,including kanamycin,ampicillin,ceftaxime and hygromycin on mycelia growth of three edible fungi Pleurotus species such as Pleurotus eryngii,Pleurotus ostreatus and Pleurotus geesteranus.The results showed that it were no significant influence that cefotaxime,kanamycin and ampicillin against three kind edible mushroom mycelial growth rate and clone morphology,moreover some treatment within the scope of experimental concentration can weakly promote the mycelial growth.Hygromycin strongly inhibited mycelial growth of three pleurotus species,and 12.5 mg/L of hygromycin can completely inhibit the growth of mycelia of P.geesteranus,and it need 50 mg/L hygromycin for achieving the aim of complete inhibition of the mycelia growth of P.eryngii and P.ostreatus.The results laid a foundation of further study on the construction of Agrobacterium Tumefaciens-mediated genetic transformation system of three Pleurotus species.

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Available abstract

The investigations were carried out that effect of four kind antibiotics,including kanamycin,ampicillin,ceftaxime and hygromycin on mycelia growth of three edible fungi Pleurotus species such as Pleurotus eryngii,Pleurotus ostreatus and Pleurotus geesteranus.The results showed that it were no significant influence that cefotaxime,kanamycin and ampicillin against three kind edible mushroom mycelial growth rate and clone morphology,moreover some treatment within the scope of experimental concentration can weakly promote the mycelial growth.Hygromycin strongly inhibited mycelial growth of three pleurotus species,and 12.5 mg/L of hygromycin can completely inhibit the growth of mycelia of P.geesteranus,and it need 50 mg/L hygromycin for achieving the aim of complete inhibition of the mycelia growth of P.eryngii and P.ostreatus.The results laid a foundation of further study on the construction of Agrobacterium Tumefaciens-mediated genetic transformation system of three Pleurotus species.

Key concepts: Mycelium, Pleurotus ostreatus, Pleurotus, Kanamycin, Mushroom, Hygromycin B, Pleurotus eryngii, Basidiocarp

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