Optimization of cellulase - assisted extraction of total flavonoids from Radix Puerariae Lobatae

To investigate the impact of cellulase on the total flavonoids extraction from Radix Puerariae Lobatae,experiment design of single factor experiments and response surface methodology(RSM) were applied.A mathematical model was developed to analyze the relationships between the main factors and the response of the extraction yield.The results of the single factor experiments showed that solvent to material ratio was not insignificant,enzyme concentration and enzymolysis time were all very significant.The results of the response surface methodology showed that the p value 0.0165 was lower than 0.01,R_(Adj)~2 were 0.8202 and Adeq.Precision was 8.671 It indicated that the model was adequate to describe the experimental value.Based on the canonical analysis,the optimum extraction conditions were obtained as follows: solvent to material ratio 12.1 ml/g,enzyme concentration 0.46%and enzymolysis time 67.9min.Under the optimized extraction conditions,the total flavonoid extraction yield was 0.184%,which was very close to the estimated value of 0.182%by regression model.