Experimental study on inhibitory effect of antihypercoagulability state enzyme of snake venom in different dosages in mice with Hepatocarcinoma

AIM: To research the inhibitory effect of antihyperc oa gulability state enzyme (AHCSE) of snake venom in different dosages in mice with Hepatocarcinoma. METHODS: Hepatic cancer H22 model in mice was performed through subcutaneous inoculation of Right-Sidedness Axilla of BALB/C mice. Different dosages of AHCSE (1, 2, and 4 mg·kg -1 ) were injected via abdominal cavity everyday for 10 days. When the tumor grew to 10 mm 3, the weight, volume, percent inhibition of tumor, spleen index and the number and the component of white blood cells were observed. RESULTS: The midd le and high dosages of AHCSE had obvious inhibition in hepatic cancer. It showed a significant dose-dependent relation in the effects of AHCSE on neoplastic we ight, neoplastic volume and splenic index. There was a linear regression between the number of leucocytes and the splenic index. CONCLUSION: AHC SE of 2- 4 mg·kg -1 has significant inhibitory effect on tumor, and sho w s dose-related effect in mice with Hepatocarcinoma. Its mechanism is presumably associated with activating immune system.