Study on the Changes of Mesenteric Microcirculation After Acute Internal Capsule Hemorrhage in Rats

Objective To observe the changes of jejunal mesenteric microcirculation before and after acute internal capsule hemorrhage in rats,and to investigate the pathogenesis of complication after cerebral hemorrhage. Methods Internal capsule hemorrhagic animal model is duplicated by injecting 100μl autologous venous blood from tail into the hind limb of internal capsule. Forty rats were randomly distributed into the sham operated group,3 h,6 h,12 h and 24 h groups after internal capsule hemorrhage. A microcirculatory microscope image analysis system was used to observe the jejunal mesentery microcirculation in rats. Volume of blood flow,wall shear rate,microthrombus of veinules,width of marginal flow and axial flow in the arterioles were measured. Results Compared with the sham operated group,blood flow of veinules in all internal capsule hemorrhage groups decreased significantly(P0.05). In 6 h,12 h and 24 h groups,wall shear rate of venules decreased markedly compared with the sham operated group(P0.05),while microthrombus increased(P0.05). Wall shear rate was inversely correlated to microthrombus(r=-0.917,P0.05).The width of marginal flow in the cerebral hemorrhage rats decreased,the ratio of width of marginal flow towidth of arteriole reduced significantly(P0.05). Conclusion The significant microvascular abnormality and blood-flow dysfunction is shown in mesenteric microcirculation of acute internal capsule hemorrhage rats,which can damage the target organs.