2012•Science and Technology of Food IndustryRequires access
Study on optimization of ultrasonic assisted extraction of polysaccharides with anti-oxidant activities from Tricholoma matsutake
Qing Gao, Lijun You, Zhao Mou-ming
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Abstract
The effect of ultrasonic power,process time and material-solvent ratio on extraction of polysaccharides with anti-oxidant activities from Tricholoma matsutake was investigated.On basis of the results of single factors test,response surface analysis was used to optimize process condition according to the response of polysaccharide yield and oxygen radical absorbance capacity value.The optimal condition of ultrasonic power,process time and material-solvent ratio was 243W,6min and 1∶16 respectively.Under the optimal conditions,the yield and oxygen radical absorbance capacity value of polysaccharides was 5.76% and 423.800μmol Trolox/g.Gel permeation chromatography analysis indicated that polysaccharides comprised two components,the molecular weight was 321356u and 19209u,the content was 50.64% and 49.36% respectively.