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Genetic Monitoring of Genomic DNAs from Guizhou Miniature Pigs BY Aflp

Feng Wu

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Abstract

We reported the application of AFLP markers to detect genetic polymorphic loci in Guizhou miniature pig and their amplified AFLP results.Their genetic construction of the population was also analysed.TenAFLP primers were used,genomic DNAs from 17 pigs were restrictive by pstⅠ,116 AFLP markers were obtained,the obtaining marker numbers of individual primer were between 2～22.The results indicate as the following:(1)AFLP marker is suitable for analysing genetic polymorphism in pig;(2)The similarty index of population in Guizhou miniature pigs was 0.866(0.760～0.967).The study provides a useful parameter with appraise genetic stability of Guizhou miniature pigs.

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What this paper is about

We reported the application of AFLP markers to detect genetic polymorphic loci in Guizhou miniature pig and their amplified AFLP results.Their genetic construction of the population was also analysed.TenAFLP primers were used,genomic DNAs from 17 pigs were restrictive by pstⅠ,116 AFLP markers were obtained,the obtaining marker numbers of individual primer were between 2～22.The results indicate as the following:(1)AFLP marker is suitable for analysing genetic polymorphism in pig;(2)The similarty index of population in Guizhou miniature pigs was 0.866(0.760～0.967).The study provides a useful parameter with appraise genetic stability of Guizhou miniature pigs.

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Available abstract

We reported the application of AFLP markers to detect genetic polymorphic loci in Guizhou miniature pig and their amplified AFLP results.Their genetic construction of the population was also analysed.TenAFLP primers were used,genomic DNAs from 17 pigs were restrictive by pstⅠ,116 AFLP markers were obtained,the obtaining marker numbers of individual primer were between 2～22.The results indicate as the following:(1)AFLP marker is suitable for analysing genetic polymorphism in pig;(2)The similarty index of population in Guizhou miniature pigs was 0.866(0.760～0.967).The study provides a useful parameter with appraise genetic stability of Guizhou miniature pigs.

Key concepts: Amplified fragment length polymorphism, Biology, Primer (cosmetics), Genetics, Population, Genetic marker, Polymorphism (computer science), Genotype

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