The Characteristics of the Antibacterial Peptides of Musca domestica Larvae Induced by Varies Microorganisms

This study investigated the characteristics of the antibacterial peptides of Musca domestica larvae that were induced by varies microorganisms.The antibacterial peptides of Musca Domestica larvae were induced by injection with three bacteria.We uses Sephadex G25 to isolate and then use the improvement method of Hult Mark and inhibition zone assay to investigate antibacterial activity of the antibacterial peptides.Capillary electrophoresis(CE) was used to analyze the differences of the peptides.We also tested the antibacterial peptides' heat stability and tolerance of pH.It was found that the antibacterial peptides induced by varies microorganisms had broad-spectrum antibacterial activity but the antibacterial activity were different in each cases.Different methods would influence the antibacterial activity results.The proteinograms obtained from CE were different with each other.All of the antibacterial peptides had heat stability and tolerance of pH.These results indicated that the antibacterial peptides' expression was related with the agents of induction.The antibacterial peptides existed in Musca Domestica larvae body originally.Induction increased the content of the antibacterial peptides and stimulated the formation of new antibacterial peptides.It was found that the antibacterial peptides induced by Shigella,Staphylococcus aureus and Salmonella typhimurium were of high contents and activity.