Chemical constituents in roots of Ilex cornuta

Objective To study the chemical constituents of the roots of Ilex cornuta.Methods The compounds were isolated and purified by silica gel,Sephadex LH-20,medium pressure column chromatography,and semi-preparative liquid chromatography,and their structures were elucidated by chemical properties and spectroscop analyses.Results Eight compounds were isolated and their structures were identified to be β-sitosterol(1),lupeol(2),betulonic acid(3),hede-ragenin(4),3β-acetoxy-28-hydroxyurs-12-ene(5),ursolic acid(6),19α-hydroxy ursolic acid(7),3β-acetoxy-ursolic acid(8),23-hydroxyl-methyl ursolate(9),heptanoic acid(10),β-daucosterol(11).Conclusion Compounds 4,5,8,and 9 are obtained from this genus for the first time,and compound 3 is obtained from this plant for the first time.