Effect of Cu~(2+),Cd~(2+) and Cr~(6+) Stress on the Anti-oxidative Enzyme Activities in Danio rerio

[Objective] The aim was to study the effect of Cu2+,Cd2+and Cr6+ on the anti-oxidative enzyme activities in Danio rerio,and discuss the toxicity effect of heavy metal on Danio rerio.[Method] Danio rerio was viewed as model organism,semi-static toxicity test was used in domestication and experiments of Danio rerio,the effects of Cu2+,Cd2+and Cr6+ on superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT) of it were discussed.[Result] At 1 d,effects of Cu2+ stress on SOD in Danio rerio was induction effect,and the stress on POD was inhibition-induction-inhibition effect,and stress on CAT was induction effect.At 7 d,effects of Cu2+ stress on SOD in Danio rerio was induction effect,and stress on POD was inhibition effect,and stress on CAT was inhibition effect.At 1 and 7 d,effects of Cd2+ stress on SOD in Danio rerio were all induction effect,and stress on POD and CAT were all inhibition effect.Moreover,the enzyme activities were changed markedly significantly at from 1 to 7d.[Conclusion] Cu2+,Cd2+and Cr6+ single stress on the anti-oxidative enzyme activities in Danio rerio were changed markedly.In short time and low-concentration exposure,Cr6+ toxicity was the most significantly;in short time and high-concentration exposure,Cd2+ toxicity was the most the most significantly;in long time exposure,Cu2+ toxicity was the most significantly.The study could provide references for heavy metal pollution on the aquatic organism toxicity mechanism.