Protective Effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside on PC12 Cells Injury Induced by H_2O_2

Objective To study the protective effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside(TSG) on the PC12 cells injury induced by H2O2.Methods All cells were divided into 5 groups:the saline control group(control group),Oxidative damage PC12 cells model group(H2O2 group) which was induced by H2O2,and TSG treated group,which oxidative damage PC12 cells model which was induced by H2O2 after given TSG 2 h at TSG 120 μg/L(TSG 120 μg/L+H2O2 group),60 μg/L(TSG 60 μg/L+H2O2 group) and 30 μg/L(TSG 30 μg/L+H2O2 group) once. The survival rate of the cells was determined by MTT method,the content of lactate dehydrogenase (LDH) was determined by ultraviolet spectrophometry and the content of malonyldialdehyde (MDA) and superoxide dismutase (SOD) activity was measured respectively by thiobarbituric aicd and xanthine oxidese method. Immunohistochemitry method were used to detect the expression of bcl-2.Results TSG reduced obviously cells injury induced by H2O2. 30~120 μg/L TSG improved the cells survival rate,reduced LDH releasing and MDA content,increased SOD activity,and decreased the expression of bcl-2 in the PC12 cells injury induced by H2O2(P0.05,P0.01). Conclusion TSG has significantly potective effect on the PC12 cells injury induced by H2O2.