Construction of recombinant adenovirus shuttle plasmid with mCD20

Objective To construct recombinant adenovirus shuttle plasmid PDC315-mCD20. Methods Mouse CD20 gene was synthesized with reverse transcription polymerase chain reaction (RT-PCR), and then was cloned into pcDNA3.1 plasmid. After identification with restriction enzyme analysis, PCR and nucleotide sequencing analysis,the PDC315-mCD20 plasmid was inserted into adenovirus shuttle plasmid, and then the recombinant plasmid were transformed into the cells of DH5a bacteria. After amplification and abstraction, the recombinant plasmid pDC315-mCD20 was obtaind. Results The recombinant plasmid was confirmed containing mCD20 gene. Conclusion We have constructed recombinant adenovirus shuttle plasmid with mCD20 successfully, which may be helpful to the next study about the construction of the adenovirus mCD20.