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Construction and identification of adenovirus shuttleplasmid pShuttle-CMV-hBMP2-IRES-hVEGF165

Yukun Zhang

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Abstract

Objective: To construct and identify the adenovirus shuttle plasmid pShuttle-CMV-hBMP2-IRES-hVEGF165.Methods: The DNA fragments of human VEGF165 gene were changed restriction sites and sub-cloned into the adenovirus shuttle plasmid pShuttle-CMV by using PCR technique.Then the human BMP2 and IRES genes were ligated into the plasmid by directional cloning method.The inserted target genes in the plasmid were verified by restriction enzyme digestion and nucleotide sequencing.Results: Construction of the adenovirus shuttle plasmid was correct.Conclusion: The adenovirus shuttle plasmid is constructed successfully.

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What this paper is about

Objective: To construct and identify the adenovirus shuttle plasmid pShuttle-CMV-hBMP2-IRES-hVEGF165.Methods: The DNA fragments of human VEGF165 gene were changed restriction sites and sub-cloned into the adenovirus shuttle plasmid pShuttle-CMV by using PCR technique.Then the human BMP2 and IRES genes were ligated into the plasmid by directional cloning method.The inserted target genes in the plasmid were verified by restriction enzyme digestion and nucleotide sequencing.Results: Construction of the adenovirus shuttle plasmid was correct.Conclusion: The adenovirus shuttle plasmid is constructed successfully.

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Available abstract

Objective: To construct and identify the adenovirus shuttle plasmid pShuttle-CMV-hBMP2-IRES-hVEGF165.Methods: The DNA fragments of human VEGF165 gene were changed restriction sites and sub-cloned into the adenovirus shuttle plasmid pShuttle-CMV by using PCR technique.Then the human BMP2 and IRES genes were ligated into the plasmid by directional cloning method.The inserted target genes in the plasmid were verified by restriction enzyme digestion and nucleotide sequencing.Results: Construction of the adenovirus shuttle plasmid was correct.Conclusion: The adenovirus shuttle plasmid is constructed successfully.

Key concepts: Plasmid, Biology, Shuttle vector, Recombinant DNA, Restriction enzyme, Molecular biology, Restriction digest, Cloning (programming)

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Construction and identification of adenovirus shuttleplasmid pShuttle-CMV-hBMP2-IRES-hVEGF165 — Research Paper | ScholarLens