The effects of Yulangsan polysaccharide on PC12 cells injury induced by Aβ_(25-35)

Objective To investigate the protective effects of YLSP on Aβ25-35 induced injury in PC12 cells. Methods Cultured PC12 cells were divided into 6 groups:control group,Aβ25-35 treated model group,positive control drug group,0.01,0.1,1.0μg·mL-1 YLSP treated groups.MTT assay was employed to analyze the PC12 cell viability.After pretreated with YLSP,the content of LDH in culture supernatant was examined by kits,The mitochondrial membrane potential(MMP) was analyzed by rhodamine 123 stain FCM.The level of reactive oxygen species(ROS) in PC12 cells was measured by dihydrohodamine 123 stain FCM. Results β-Amyloid peptide 25-35(Aβ25-35) induced a decrease in viability(P0.01) and an increase of LDH release of PC12 cells(P0.01) along with dissipation of MMP(P0.01) as well as well overproduction of ROS(P0.01).When PC12 cells were co-treated with YLSP and Aβ25-35,a decrease in viability of PC12 cells induced by 10μM Aβ25-35 was blocked by YLSP(P0.01).YLSP also decreased the release of LDH(P0.05) and inhibited the dissipation of MMP(P0.05 or P0.01) and overproduction of ROS(P0.05 or P0.01). Conclusion YLSP can protect PC12 cells from injury induced by Aβ25-35,which may be associated with the overproduction of ROS induced by Aβ25-35.