Isolation and structure identification of chemical constituents from buds of Lonicera ruprechtiana Regel

Objective To explore the medicinal values of the buds of Lonicera ruprechtiana Regel.by analyzing its chemical constituents. Methods The buds of Lonicera ruprechtiana Regel.were extracted with 70% ethanol,the extractives were purified with macroporous resin and isolated with silica gel and ODS column chromatography.NMR,UV,IR Spectrometers were used to obtain spectroscopic data.Results According to the single spot in TLC,five compounds were isolated from the buds of Lonicera ruprechtiana Regel.,and their structures were identifited through physico-chemical constants and spectroscopic analysis.Five compounds were isolated from the buds of Lonicera ruprechtiana Regel.,and they are daucosterol (Ⅰ);7S-O-methylmorroniside(Ⅱ);neohesperidin(Ⅲ);benzyl-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside (Ⅳ);6'-O-β-D-apiofuranosyl sweroside (Ⅴ).Conclusion All these compounds are isolated from the buds of Lonicera ruprechtiana Regel.for the first time.