Study on biofilm-formation ability of clinical strains of Acinetobacter baumannii

Objective To study comparatively biofilm-formation ability of Acinetobacter baumannii and analyze some influencing factors which affected the biofilm formation of Acinetobacter baumannii.Method The biofilm formation ability of 51 strains resistant to all traditional antibiotics were determined by constructing biofilms on 24-well polystyrene plates.The biofilm formation ability of 20 strains Acinetobacter baumannii isolated from Lower respiratory tract and 20 strains separated from sterile body fluid were determined.And abaI genes associated with biofilm formation of Acinetobacter baumannii were detected as well.Result 68.6%(35/51) strains resistant to all traditional antibiotics could form biofilms.50%(10/20) strains from lower respiratory tract could form strong biofilms and 5%(1/20) strains from sterile body fluid could form biofilms.78.0%(71/91) of all strains contained abaI genes.Conclusion The Acinetobacter baumannii strains isolated from lower respiratory tract can form strong biofilm.The abaI genes were prevalent in clinically strains of Acinetobacter baumannii.And the influence of biofilm should been taken into consideration when patients suffering from Acinetobacter baumannii infection accepted treatment.