Effect of nedaplatin on cervical cancer HeLa cells and SiHa cells

Objective: To compare the inhibiting effect of nedaplatin in vitro on human cervical adenocarcinoma HeLa cells and cervical squamous carcinoma SiHa cells and explore the mechanism.Methods: HeLa cells and SiHa cells were treated with nedaplatin(2.5-40 μg/ml) for 24,48 and 72 hours,respectively,MTT method was used to detect the inhibition rate,and then the median inhibitory concentration(IC50) was calculated;flow cytometry was used to observe the apoptotic rate and change of cycles;semiquantitative PCR was used to analyze the changes of expressions of Caspase 3,Caspase 9 and matrix metal proteinase-2(MMP-2).Results: Nedaplatin inhibited cervical cancer HeLa cells and SiHa cells,showing a time-dose-dependent manner,the inhibition rate of nedaplatin on HeLa cells was higher than that on SiHa cells;the results of flow cytometry showed that the apoptotic rates of HeLa cells and SiHa cells increased with the increase of concentration,the apoptotic rate of HeLa cells was statistically significantly higher than that of SiHa cells under the same concentration and action time(P 0.05),the proportion of cells at S phase increased,and the proportion of cells at G0 / G1 phase decreased,the change of proportion of cells at G2 / M phase was not significant;compared with control group,the expression levels of Caspase 3 and Caspase 9 increased,while the expression level of MMP-2 decreased.Conclusion: Nedaplatin can inhibit the proliferation of cervical cancer HeLa cells and SiHa cells,application of nedaplatin in vitro has more stronger inhibiting effect on HeLa cells.The mechanism is correlated with inducing apoptosis,cell cycle arrest,up-regulation of Caspase3 and Caspase9;down-regulation of MMP-2 may be helpful to reduce the invasive ability of cervical cancer cells.