SRAP Analysis of the Genetic Relationship of Prunus L.
Liu Y
Abstract
Liu Y
Abstract
Providing a theoretical basis for the identification of ‘ Bashan Cuili' and protection and utilization of other crisp varieties in Dazhou, the sequence related amplified polymorphism was used to analyse the genetic relationship of the Bashan Cuili and the excellent regional resources in Dazhou. Twelve primer pairs out of 180 pairs of sequence related amplified polymorphism(SRAP) markers which were of high polymorphism and showed clear bands were used to analyze the genetic diversity of 22 samples, which generating 103 bands with 64 bands(60.34%) as polymorphic ones. Each primer combination amplified loci was 5.42. A dendrogram was constructed based on the SRAP data using UPGMA cluster method. The 22 samples of plum were classified into 3 major groups when the similarity coefficient was at 0.68. The clustering results and fruit shape was basically corresponded, and was related with resource distribution and mature phase.
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Providing a theoretical basis for the identification of ‘ Bashan Cuili' and protection and utilization of other crisp varieties in Dazhou, the sequence related amplified polymorphism was used to analyse the genetic relationship of the Bashan Cuili and the excellent regional resources in Dazhou. Twelve primer pairs out of 180 pairs of sequence related amplified polymorphism(SRAP) markers which were of high polymorphism and showed clear bands were used to analyze the genetic diversity of 22 samples, which generating 103 bands with 64 bands(60.34%) as polymorphic ones. Each primer combination amplified loci was 5.42. A dendrogram was constructed based on the SRAP data using UPGMA cluster method. The 22 samples of plum were classified into 3 major groups when the similarity coefficient was at 0.68. The clustering results and fruit shape was basically corresponded, and was related with resource distribution and mature phase.
Key concepts: UPGMA, Dendrogram, Genetic diversity, Biology, Genetic relationship, Polymorphism (computer science), Similarity (geometry), Primer (cosmetics)