Determination of Vitamin A and E in Fortified Foods by Cold Saponification-HPLC Method

Objective To establish a cold saponification-HPLC method for the determination of vitamin A and vitamin E in fortified foods.Method Samples were saponified over night,adjusted to pH 6.0-7.5 with acetic acid and determined by HPLC.Chromatographic conditions:using SUPERIOREX ODS column (4.6 mm × 250 mm,5 μm) with a mobile phase of methanol and water (94+ 6).Results The linearity ranges were 0.25-3.00 μg /ml for vitamin A and 10.0150.0 μg /ml for vitamin γ-E,δ-E and α-E.The correlation coefficients were 0.999 7-0.999 9.The qualitative detection limit was 0.40 μg /g for vitamin A,5.0 μg /g for vitamin γ-E and δ-E and 8.0 μg /g for vitamin α-E.The quantitative detection limits of the method were 1.30 μg /g for vitamin A,16.7 μg /g for vitamin γ-E and δ-E,and 26.7 μg /g for vitamin α-E.The average recoveries of adding standards were 77.1%-105.0% and the relative standard deviation (RSD) of the method was less than 5%.Conclusion The method was simple,fast,accurate and reproducible and could be used to determine the contents of vitamin A and vitamin E in vitamin fortified foods.