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2008•Hebei Journal of Forestry and Orchard ResearchRequires access

Micropropagation of Bothrocaryum controversum Hemsl.

Wang Chen

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Abstract

This experiment was conducted to investigate in vitro micropropagation of Bothrocaryum controversum Hemsl.with the apical buds excised from the mature elite plants.The results showed that the optimum induction medium was woody plant medium(WPM) supplemented with 0.5 mg/L 6-benzylaminopurine(BA),0.2 mg/L indole-3-butyric acid(IBA) and 1 mg/L gibberellin(GA3);and the highest shoot proliferation rate of 4.4 times was obtained on the medium M(the concentration of CaCl2·2H2O in Murashige and Skoog(MS) medium was 1 200 mg/L) supplemented with 1 mg/L BA,0.2 mg/L IBA and 2 mg/L GA3;and 1/2M with 3 mg/L IBA was the optimum shoot rooting medium,on which the shoots cultured 10 days in dark then transferred to the light condition had the highest rooting efficiency(60.0%).

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What this paper is about

This experiment was conducted to investigate in vitro micropropagation of Bothrocaryum controversum Hemsl.with the apical buds excised from the mature elite plants.The results showed that the optimum induction medium was woody plant medium(WPM) supplemented with 0.5 mg/L 6-benzylaminopurine(BA),0.2 mg/L indole-3-butyric acid(IBA) and 1 mg/L gibberellin(GA3);and the highest shoot proliferation rate of 4.4 times was obtained on the medium M(the concentration of CaCl2·2H2O in Murashige and Skoog(MS) medium was 1 200 mg/L) supplemented with 1 mg/L BA,0.2 mg/L IBA and 2 mg/L GA3;and 1/2M with 3 mg/L IBA was the optimum shoot rooting medium,on which the shoots cultured 10 days in dark then transferred to the light condition had the highest rooting efficiency(60.0%).

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Available abstract

This experiment was conducted to investigate in vitro micropropagation of Bothrocaryum controversum Hemsl.with the apical buds excised from the mature elite plants.The results showed that the optimum induction medium was woody plant medium(WPM) supplemented with 0.5 mg/L 6-benzylaminopurine(BA),0.2 mg/L indole-3-butyric acid(IBA) and 1 mg/L gibberellin(GA3);and the highest shoot proliferation rate of 4.4 times was obtained on the medium M(the concentration of CaCl2·2H2O in Murashige and Skoog(MS) medium was 1 200 mg/L) supplemented with 1 mg/L BA,0.2 mg/L IBA and 2 mg/L GA3;and 1/2M with 3 mg/L IBA was the optimum shoot rooting medium,on which the shoots cultured 10 days in dark then transferred to the light condition had the highest rooting efficiency(60.0%).

Key concepts: Shoot, Micropropagation, Murashige and Skoog medium, Gibberellic acid, Gibberellin, Explant culture, Botany, Biology

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