Determination of Catalpol Content in Radix Rehmanniae from Genuine Producing Area by Near Infrared Spectroscopy
Caixia Xie
Abstract
Caixia Xie
Abstract
Objective:The quantitative analysis model for determination of the content of catalpol in Radix Rehmanniae from genuine producing area was established using near-infrared spectroscopy.Method:The catalpol content was determined by HPLC.The near-infrared model was established combined the near-infrared spectroscopy with the value determined by HPLC.And then predict the model with verification.Result:The correlation coefficient of calibration(R2) was 0.993 83.Root mean square error of cross validation(RMSEC) was 0.087 5;Root mean square error of prediction(RMSEP) was 0.032 0.Conclusion:It was feasible to determine the catalpol content in Radix Rehmanniae and it provided a rapid convenient method for quality control of Radix Rehmanniae.
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Objective:The quantitative analysis model for determination of the content of catalpol in Radix Rehmanniae from genuine producing area was established using near-infrared spectroscopy.Method:The catalpol content was determined by HPLC.The near-infrared model was established combined the near-infrared spectroscopy with the value determined by HPLC.And then predict the model with verification.Result:The correlation coefficient of calibration(R2) was 0.993 83.Root mean square error of cross validation(RMSEC) was 0.087 5;Root mean square error of prediction(RMSEP) was 0.032 0.Conclusion:It was feasible to determine the catalpol content in Radix Rehmanniae and it provided a rapid convenient method for quality control of Radix Rehmanniae.
Key concepts: Catalpol, Radix (gastropod), Correlation coefficient, Near-infrared spectroscopy, Chemistry, Infrared spectroscopy, Calibration, Chromatography