Empirical treatment study of Xuebijing injection on acute paraquat poisoning

Objective To approach the mechanism of Xuebijing injection treat acute paraquat poisoning.Methods All 18 healthy adult wistar rats were randomized assigned into paraquat 250 mg/kg poisoning group,paraquat 250 mg/kg poisoning + Xuebijing injection(1.72 g crude drug /kg)treat groups, paraquat 250 mg/kg poisoning + Xuebijing injection(0.86 g crude drug /kg)treat group.Paraquat were given by gastric tube.Xuebijing injection were given through rat tail vein 1 h later.Arteriovenous blood was drawn 8 h later and lung tissue was remained.Arterial blood gas analysis and alanine aminotrans ferase(ALT),alkaline phosphatase(AKP),blood urea nitrogen(BUN),creatinine(Cr) were determined.Cytokin TNF-α,IL-6,IL-10,IL-1β,platelet-derived growth factor(PDGF),insulin-like growth factor-1(IGF-1) were also determined.And we observed the pathological changed.Results The PaO_2,PaCO_2 and pH of Xuebijing injection groups were better than those of non-treat group(P0.05 or 0.01),and ALT,Cr of Xuebijing injection groups were lower(P0.05).There was no significant differerce of TNF-α,IL6 between treatment and non-treatment groups(P0.05).Cytokine IL-10,IL-1β,PDGF、IGF-1 of treatment group were lower than that of non-treatment group(P0.05).The pathology discovered that the lung tissue was engorged, congestive in non-treatment groups,and many phlegmasia cells were infiltrated in lung tissue.The changes was obviously lighter in treatment groups.Conclusion Xuebijing injection could inhibit activities of some cytokines and protect the organ injury caused by paraquat.