Influence of xuebijing injection on IL-1,IL-6 and TNF-α in rats caused by acute paraquat intoxication

Objective To observe the influence of xuebijing injection on IL-1,IL-6,TNF-α in acute paraquat rats,and discuss whether xuebijing injection can inhibit the inflammatory response cased by paraquat poisoning.Methods 30 male Wister rats were randomly divided into control group,poisoning control group,xuebijing treatment group,10 rats each group.Control group was given saline(10 mL/kg i.p.);poisoning control group and xuebijing treatment group were given paraquat(20 mg/kg i.p.).After 30 minutes,control group and poisoning control group were given saline(10 mL/kg)once a day by intraperitoneal injection,xuebijing treatment group was given xuebijing(10 mL/kg)once a day by intraperitoneal injection.To collect 1.5 mL blood from inner and canthal vein on the 3rd day,7th day,14th day.To separate serum and detect the expression level of IL-1,IL-6,TNF-α and analyze the experimental datas and discuss the correlation of the three groups.Results The expression levels of IL-1,IL-6,TNF-α of poisoning control group and xuebijing treatment group were obviously higher than those of control group(P0.001).The expression levels of IL-1,IL-6,TNF-α of xuebijing treatment group were lower than those of poisoning control group(P0.001).Conclusion Inflammatory factors increase in paraquat poisoning,which can cause multiple organ failures,but xuebijing can reduce the injury caused by inflammatory mediators and inhibit the increasing of IL-1,IL-6 and TNF-α.Which provide a basis for future clinical therapy.