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2006•Zhongguo redai yixueRequires access

Observation on the optimum time for harvesting hematopoietic cells from cord blood after culture in vitro.

Wenjian Mo

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Abstract

Objective To explore the optimal time for harvesting cord blood hematopoietic cells cultured in vitro by colony multiplication. Methods Mononuclear cells separated from cord blood were cultured in a serum-free system supplemented with cytokines or human bone marrow stromal cells of combination of them. CFU-MK,BFU-E and CFU-Gm were assessed by semisolid culture assay on d0,d6,d10 and d14 for determination of progenitor unmber. Results The quantity of CFU-MK,BFU-E and CFU-Gm on d10 after culture were significantly higher than that of other culture time (P0.05). The number of colony forming units were higher in the groups supported with human bone marrow stromal cells (P0.05). Conclusion The optimal time for harvesting the mononuclear cells is on the tenth day after in vitro culture of cord blood hematopoietic cells. Bone marrow stromal cells is capable of maintaining the activity of hematopoietic cells cultured in vitro.

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Objective To explore the optimal time for harvesting cord blood hematopoietic cells cultured in vitro by colony multiplication. Methods Mononuclear cells separated from cord blood were cultured in a serum-free system supplemented with cytokines or human bone marrow stromal cells of combination of them. CFU-MK,BFU-E and CFU-Gm were assessed by semisolid culture assay on d0,d6,d10 and d14 for determination of progenitor unmber. Results The quantity of CFU-MK,BFU-E and CFU-Gm on d10 after culture were significantly higher than that of other culture time (P0.05). The number of colony forming units were higher in the groups supported with human bone marrow stromal cells (P0.05). Conclusion The optimal time for harvesting the mononuclear cells is on the tenth day after in vitro culture of cord blood hematopoietic cells. Bone marrow stromal cells is capable of maintaining the activity of hematopoietic cells cultured in vitro.

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Available abstract

Objective To explore the optimal time for harvesting cord blood hematopoietic cells cultured in vitro by colony multiplication. Methods Mononuclear cells separated from cord blood were cultured in a serum-free system supplemented with cytokines or human bone marrow stromal cells of combination of them. CFU-MK,BFU-E and CFU-Gm were assessed by semisolid culture assay on d0,d6,d10 and d14 for determination of progenitor unmber. Results The quantity of CFU-MK,BFU-E and CFU-Gm on d10 after culture were significantly higher than that of other culture time (P0.05). The number of colony forming units were higher in the groups supported with human bone marrow stromal cells (P0.05). Conclusion The optimal time for harvesting the mononuclear cells is on the tenth day after in vitro culture of cord blood hematopoietic cells. Bone marrow stromal cells is capable of maintaining the activity of hematopoietic cells cultured in vitro.

Key concepts: Haematopoiesis, Cord blood, Bone marrow, Stromal cell, Peripheral blood mononuclear cell, CFU-GM, In vitro, Colony-forming unit

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