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2003•Zhongguo yufang shouyi xuebaoRequires access

Cloning and Sequncing of the Chicken Interleukin-18

Wei Xing Pan

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Abstract

Interleukin 18(IL_18)is a novel potent inducer of IFN_γ production and plays an important role in Th1 type immune response.In this study,specific primers for chicken inteleukin_18(chIL_18)cDNA have been designed and synthesized according to the previously reported nucleic acid sequence of chicken interleukin_18 cDNA.Using total RNA from ConA_stimulated chicken splenocytes,chicken IL_18 cDNA encoding mature protein was amplified by reverse transcription polymerase chain reaction(RT_PCR).Sequence analysis indicated that there is one nucleotide different from the pubilshed chIL_18 cDNA sequence in 482(T_C).It is the first chicken IL_18 cDNA that has been cloned in China and this study has paved a way for future study about the expression and biological function of chIL_18.

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What this paper is about

Interleukin 18(IL_18)is a novel potent inducer of IFN_γ production and plays an important role in Th1 type immune response.In this study,specific primers for chicken inteleukin_18(chIL_18)cDNA have been designed and synthesized according to the previously reported nucleic acid sequence of chicken interleukin_18 cDNA.Using total RNA from ConA_stimulated chicken splenocytes,chicken IL_18 cDNA encoding mature protein was amplified by reverse transcription polymerase chain reaction(RT_PCR).Sequence analysis indicated that there is one nucleotide different from the pubilshed chIL_18 cDNA sequence in 482(T_C).It is the first chicken IL_18 cDNA that has been cloned in China and this study has paved a way for future study about the expression and biological function of chIL_18.

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Available abstract

Interleukin 18(IL_18)is a novel potent inducer of IFN_γ production and plays an important role in Th1 type immune response.In this study,specific primers for chicken inteleukin_18(chIL_18)cDNA have been designed and synthesized according to the previously reported nucleic acid sequence of chicken interleukin_18 cDNA.Using total RNA from ConA_stimulated chicken splenocytes,chicken IL_18 cDNA encoding mature protein was amplified by reverse transcription polymerase chain reaction(RT_PCR).Sequence analysis indicated that there is one nucleotide different from the pubilshed chIL_18 cDNA sequence in 482(T_C).It is the first chicken IL_18 cDNA that has been cloned in China and this study has paved a way for future study about the expression and biological function of chIL_18.

Key concepts: Complementary DNA, Biology, Molecular biology, Rapid amplification of cDNA ends, Cloning (programming), Nucleic acid, Reverse transcription polymerase chain reaction, Nucleic acid sequence

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