ScholarLens Back to search
2009•Zhongguo shengwuzhipinxue zazhiRequires access

Effect of Stimulation with LPS on Expression of High Mobility Group Box1 in Murine Peritoneal Macrophages

Wei Cheng-guo

Open publisher page 0 citations

Abstract

Objective To observe the effect of stimulation with LPS on the expression of high mobility group box1(HMGB1) in murine peritoneal macrophages.Methods The peritoneal macrophages of normal mice were isolated and stimulated with LPS for 4,8,12,18,24 and 36 h respectively,then determined for transcription level of HMGB1 mRNA by RT-PCR and for expression level of HMGB1 protein by Western blot.The time-response relationship between LPS stimulation and HMGB1 release was observed.Results The transcription level of HMGB1 mRNA showed no significant change 4～18 h,while increased significantly 24 h and showed significant difference with those of control 24 and 36 h after LPS stimulation.However,the expression level of HMGB1 protein increased significantly from 18 h until 36 h after LPS stimulation.Conclusion LPS showed a time-dependent promoting effect on the expression of HMGB1 in murine peritoneal macrophages.

About this research paper

What this paper is about

Objective To observe the effect of stimulation with LPS on the expression of high mobility group box1(HMGB1) in murine peritoneal macrophages.Methods The peritoneal macrophages of normal mice were isolated and stimulated with LPS for 4,8,12,18,24 and 36 h respectively,then determined for transcription level of HMGB1 mRNA by RT-PCR and for expression level of HMGB1 protein by Western blot.The time-response relationship between LPS stimulation and HMGB1 release was observed.Results The transcription level of HMGB1 mRNA showed no significant change 4～18 h,while increased significantly 24 h and showed significant difference with those of control 24 and 36 h after LPS stimulation.However,the expression level of HMGB1 protein increased significantly from 18 h until 36 h after LPS stimulation.Conclusion LPS showed a time-dependent promoting effect on the expression of HMGB1 in murine peritoneal macrophages.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To observe the effect of stimulation with LPS on the expression of high mobility group box1(HMGB1) in murine peritoneal macrophages.Methods The peritoneal macrophages of normal mice were isolated and stimulated with LPS for 4,8,12,18,24 and 36 h respectively,then determined for transcription level of HMGB1 mRNA by RT-PCR and for expression level of HMGB1 protein by Western blot.The time-response relationship between LPS stimulation and HMGB1 release was observed.Results The transcription level of HMGB1 mRNA showed no significant change 4～18 h,while increased significantly 24 h and showed significant difference with those of control 24 and 36 h after LPS stimulation.However,the expression level of HMGB1 protein increased significantly from 18 h until 36 h after LPS stimulation.Conclusion LPS showed a time-dependent promoting effect on the expression of HMGB1 in murine peritoneal macrophages.

Key concepts: HMGB1, Stimulation, Western blot, High-mobility group, Messenger RNA, Lipopolysaccharide, Transcription (linguistics), Andrology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effect of Stimulation with LPS on Expression of High Mobility Group Box1 in Murine Peritoneal Macrophages — Research Paper | ScholarLens