Effect of Alpha Lipoic Acid on Apoptosis of Cardiomyocyte inII Diabetic Rats

Objective To investigate the effect of Alpha Lipoic Acid(A-LA) on apoptosis of cardiomyocyte in Ⅱdiabetic rat.Methods SD rats were randomly divided into five groups: control group,diabetic group,A-LA low,medium and high dose groups(n=8).Control group was given ordinary diet,and experimental group was given diet of high fat and sugar;after a month experimental group was injected small doses of streptozotocin to the intra-peritoneal of a urine at 30 mg/kg,thereby establishing type Ⅱdiabetes model.A-LA low,medium and high dose groups were separately given 15,30,60 mg/(kg·d) for 12 weeks.Then testing blood sugar and blood fat,the activities of SOD and GSH-PX were also measured with serum and heart tissue.Apoptosis was detected by TUNEL.Results Compared with control group,diabetes model rats' blood sugar and TG,TC,and LDL-C increased significantly(P0.01),while HDL-C reduced significantly(P0.01),and the activities of SOD and GSH-PX were significantly reduced(P0.01);the rate of cell apoptosis was increased.Compared with the diabetic group,as for A-LA experimental group,all indexes above had been improved.The medium group's improvement is clear.Blood glucose and TG,TC,LDL-C reduced significantly(P0.05),HDL-C increased significantly(P0.05),the activities of SOD and GSH-PX were improved(P0.01);the rate of cell apoptosis was reduced.Conclusion Alpha Lipoic Acid has a protective effect on cardiac muscle in diabetic rats,the mechanism may be related to the inhibition of oxidative stress with low blood sugar and low blood fat level,and then the cell apoptosis rate is reduced.