Study of the Effect of Triptolide on Ovarian Cancer Cell Line A2780

Objective:To evaluate the value and significance of triptolide as an antitumor drug.The inhibitive effect of triptolide and the synergistic effect of triptolide combined with cisplatin and comparison of the effect between triptolide and cisplatin on ovarian cancer cell line A2780 are investigated.Methods:Methyl thiazolyl tetrazolium(MTT)assay was used to examined the inhibitive effect of triptolide on ovarian cancer cells' proliferation in vitro,the cells' proliferation affected by triptolide was observed by microscope,and the cells' apoptosis after treatment with triptolide was observed by FCM.Results:1)Triptolide had significant effect on ovarian cancer cell line A2780 in a dose-dependent and time-dependent manner(P0.05);At lower concentration(5 mg/L,0.5 mg/L,0.005 mg/L),the inhibitive rate was 80%,60% and 40% respectively.2)Comparing with cisplatin,triptolide had more significant inhibitive effect on A2780 at low concentration(5 mg/L)(P0.05),but at high concentration(5 mg/L)their inhibitive effects were not statistically significantly different(P0.05);and the IC50 of triptolide was less than that of cisplatin in 24 h,48 h,and 72 h after treatment.3)At low concentration(5 mg/L),the inhibitive rate of combination triptolide with cisplatin was higher than that of cisplatin alone,but lower than that of triptolide alone.And the inhibitive rates increased with the prolongation of treated time,especially when the treated time was longer than 48 h.4)Apoptosis existed in A2780 after treatment with triptolide(P0.05).Conclusion:Triptolide has significant suppressive effect on ovarian cancer cell line A2780 in a dose-dependent and time-dependent manner(P0.05),and it works even in a low concentration(5 mg/L),At lower concentration,the inhibitive rate of triptolide is higher than that of cisplatin.The inhibitive rate of combined triptolide and cisplatin increases with the prolongation of affected time.The anti-tumor effect of triptolide may be achieved by inducing cells' apoptosis.