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2011•Chinese Journal of Drug Application and MonitoringRequires access

Studies on class I integron carriage among 100 Escherichia coli isolates from clinical children

Su Mei

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Abstract

Objective:To investigate the occurrence and distribution of class I integron among 100 Escherichia coli isolates from clinical children.Methods:Antibacterial susceptibility testing was conducted by agar dilution test according to NCCLS.ClassⅠintegrase gene PCR was performed to detect the presence of integron.Results:The positive rate of class Ⅰ integrase and integron were 68% and 65% respectively.The size of class Ⅰ integrons ranged from 772 bp to 2360 bp.Each of the 100 isolates had 1 or 2 class Ⅰ integrons.The main order of inserted gene cassettes was dfrA17 + aadA5 and dfrA12 + aadA2.Conclusion:Class Ⅰ integrons are widespread in isolates of multidrug resistant E.coli.Integrons are important molecular mechanism in the development of multidrug resistance.The results stressed the need for surveillance of bacteria in genetic levels.

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Objective:To investigate the occurrence and distribution of class I integron among 100 Escherichia coli isolates from clinical children.Methods:Antibacterial susceptibility testing was conducted by agar dilution test according to NCCLS.ClassⅠintegrase gene PCR was performed to detect the presence of integron.Results:The positive rate of class Ⅰ integrase and integron were 68% and 65% respectively.The size of class Ⅰ integrons ranged from 772 bp to 2360 bp.Each of the 100 isolates had 1 or 2 class Ⅰ integrons.The main order of inserted gene cassettes was dfrA17 + aadA5 and dfrA12 + aadA2.Conclusion:Class Ⅰ integrons are widespread in isolates of multidrug resistant E.coli.Integrons are important molecular mechanism in the development of multidrug resistance.The results stressed the need for surveillance of bacteria in genetic levels.

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Available abstract

Objective:To investigate the occurrence and distribution of class I integron among 100 Escherichia coli isolates from clinical children.Methods:Antibacterial susceptibility testing was conducted by agar dilution test according to NCCLS.ClassⅠintegrase gene PCR was performed to detect the presence of integron.Results:The positive rate of class Ⅰ integrase and integron were 68% and 65% respectively.The size of class Ⅰ integrons ranged from 772 bp to 2360 bp.Each of the 100 isolates had 1 or 2 class Ⅰ integrons.The main order of inserted gene cassettes was dfrA17 + aadA5 and dfrA12 + aadA2.Conclusion:Class Ⅰ integrons are widespread in isolates of multidrug resistant E.coli.Integrons are important molecular mechanism in the development of multidrug resistance.The results stressed the need for surveillance of bacteria in genetic levels.

Key concepts: Integron, Carriage, Integrase, Escherichia coli, Microbiology, Gene cassette, Agar dilution, Agar Dilution Method

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