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THE STUDY ON CLASS 1 INTEGRONS IN MEDIATION OF ANTIBIOTIC RESISTANCE IN ISOLATES OF ESCHERICHIA COLI FROM BLOOD INFECTION.

Liu Quan-xu

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Abstract

Objective:To explore the molecular mechanism of class 1 integrons in mediation of antibiotic resistance in isolates of Escherichia coli from blood infection.Methods:The antimicrobial susceptibility test of 16 Escherichia coli isolates were performed by Microscan WalkAway40 and the disk diffusion assay.Class 1 integrons were detected by PCR with total DNA.The purified amplicons of class 1 integrons were sequenced,and gene cassettes were identified.Results:8 isolates carried class 1 integrons.The sizes of class 1 integrons were 600bp,1700bp and 2500bp respectively.600bp integron carried gene cassette dfr2d,and 1700bp integron carried gene cassette dfr17-aadA5.Conclusion:Integrons play an important role in mediation of antibiotic resistance.

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Objective:To explore the molecular mechanism of class 1 integrons in mediation of antibiotic resistance in isolates of Escherichia coli from blood infection.Methods:The antimicrobial susceptibility test of 16 Escherichia coli isolates were performed by Microscan WalkAway40 and the disk diffusion assay.Class 1 integrons were detected by PCR with total DNA.The purified amplicons of class 1 integrons were sequenced,and gene cassettes were identified.Results:8 isolates carried class 1 integrons.The sizes of class 1 integrons were 600bp,1700bp and 2500bp respectively.600bp integron carried gene cassette dfr2d,and 1700bp integron carried gene cassette dfr17-aadA5.Conclusion:Integrons play an important role in mediation of antibiotic resistance.

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Available abstract

Objective:To explore the molecular mechanism of class 1 integrons in mediation of antibiotic resistance in isolates of Escherichia coli from blood infection.Methods:The antimicrobial susceptibility test of 16 Escherichia coli isolates were performed by Microscan WalkAway40 and the disk diffusion assay.Class 1 integrons were detected by PCR with total DNA.The purified amplicons of class 1 integrons were sequenced,and gene cassettes were identified.Results:8 isolates carried class 1 integrons.The sizes of class 1 integrons were 600bp,1700bp and 2500bp respectively.600bp integron carried gene cassette dfr2d,and 1700bp integron carried gene cassette dfr17-aadA5.Conclusion:Integrons play an important role in mediation of antibiotic resistance.

Key concepts: Integron, Gene cassette, Escherichia coli, Biology, Microbiology, Antibiotic resistance, Amplicon, Gene

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THE STUDY ON CLASS 1 INTEGRONS IN MEDIATION OF ANTIBIOTIC RESISTANCE IN ISOLATES OF ESCHERICHIA COLI FROM BLOOD INFECTION. — Research Paper | ScholarLens