Construction and expression of eukaryotic expression vector bearing fusion gene of human IL-2 cDNA gene and Fc gene

Objective:To construct and express a recombinant eukaryotic expression vector bearing fusion gene of human IL-2 cDNA gene and Fc fragment. Methods:Technique of splicing by overlapping extension and two times PCR were used, and fusion gene fragment was obtained and cloned into pGEM-T Easy TA cloning vector to get suited enzymed sites. Recombinant eukaryotic expression vector pcDNA 3.1 IL-2/Fc was constructed by double adhesive terminal ligation. Then the recombinant vector was transferred into SP 2/0 cells using Lipofectamine. Results:The recombinant vector was identified by digestion with restriction enzymes and confirmed by DNA sequencing analysis. And exocrine expression of the fusion gene in eukaryotic cells was detected by ELASA method. Conclusion:The relative efficient expression of the fusion gene in SP 2/0 cells might provide an experimental basis for improving the efficiency of DNA vaccine.