A new method of bone marrow biopsy specimen preservation for immunohistochemistry

In order to perform immunohistochemistry on bone marrow biopsy specimen sections, the new method of freeze-substitution and subsequent low-temperature plastic embedding was studied. The results showed that the morphologic preservation of bone marrow tissue is excellent and all antigens of blood cells, especially labile lymphocyte surface markers were well preserved, which had an accurate localization, no diffusion, and absence of background staining. This is an ideal method for bone marrow biopsy specimen preservation for immunohistochemistry. It is also suitable for processing of needle and endoscopic biopsy specimens.