Single nucleotide polymorphysims of CYP2D6 gene G4268C and ERCC1 gene C8092A and their genetic susceptibility to lung cancer

Objective:To evaluate the impact of single nucleotide polymorphysims of CYP2D6 gene G4268C and ERCC1 gene C8092A on the lung cancer occurrence in Liaoning province.Methods:A case-control study was conducted included 200 lung cancer patients and 200 control subjects.KI method was used to isolate human genomic DNA from whole blood.PCR-RFLP was used to identify CYP2D6 gene G4268C,C188T and ERCC1 gene C8092A genotyes of polymorphysims.Results:The distribution frequency of G4268/G,G4268/C and C4268/C were 1.50%,58.00%,40.50% in lung cancer group and 2.50%,44.00%,53.50% in control group.The individuals who carried non-C4268/C genotype had a 1.73-fold increased risk with lung cancer than those who carried C4268/C genotype(95%CI=1.02-2.95),especially to lung adenocarcinoma [OR=2.75(95%CI=1.27-5.94)].After stratification analysis according to smoking,it revealed that non-C4268/C genotype was associated with significantly increased risk in non-smokers and light-smokers subgroups,with OR=2.09(95%CI=1.09-4.25) and 3.41(95%CI=1.24-9.93)respectively.The individuals who carried non-A8092/A genotype had a 0.98-fold increased risk with lung cancer than those who carried A8092/A genotype(95%CI=0.52-2.17).Conclusion:C4268/C genotype,as protective factors in non-smokers of light-smokers subgroups,may help decreasing the susceptibility to lung cancer subjects.ERCC1 gene C8092A polymorphysim had no association with lung cancer.